Skip to main content
. 2007 Jan 26;189(7):2637–2645. doi: 10.1128/JB.01798-06

FIG. 2.

FIG. 2.

Sporulation phenotypes of B. anthracis (A) and B. subtilis (B) strains expressing the Spo0E-like proteins from the multicopy plasmid pHT315. Streaks in panel A are strains carrying the following plasmids: sector 1, pHT315; sector 2, pHT315-YisI; sector 3, pHT315-0046; sector 4, pHT315-1877; sector 5, pHT315-2416; sector 6, pHT315. Strains in panel B carry the following plasmids: sector 1, pHT315; sector 2, pHT315-1877; sector 3, pHT315-2416; sector 4, pHT315-YisI; sector 5, pHT315-0046. Sporulation on agar plates results in a level of opacity of the streak that is associated with the efficiency of spore formation. The more opaque the streak is, the whiter it appears in a black-and-white picture. Sporulation-deficient, and thus transparent, streaks, as in sector 5 in panel A and sector 2 in panel B, have a gray appearance. Strains were streaked on SM plates and incubated at 37°C for 48 h.