FIG. 3.

Effect of iron availability on fur::luxAB (pHL01) expression in the wild-type and in the Δfur mutant V. vulnificus. Wild type (WT) and Δfur mutant V. vulnificus carrying pHL01 were grown in LBS medium supplemented with 5 μg ml⁻¹ tetracycline. Iron in the medium was depleted by adding 0.2 mM 2,2′-dipyridyl to the early exponential cultures (OD₆₀₀ of 0.1). The fur::luxAB activities were normalized by dividing the number of RLUs by the OD₆₀₀ value. The fur::luxAB activities of four independent cultures at exponential phase (OD₆₀₀ of 0.5) were averaged and are indicated with their standard deviations.