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Expression and processing of procoat protein double-cysteine mutants. E. coli K38 cells bearing plasmids expressing double mutants with one cysteine residue at position 31 (A), 32 (B), or 33 (C) and a second cysteine residue at the positions indicated below the lanes were induced for 3 h, acid precipitated, and analyzed by SDS-PAGE and Western blotting.
