TABLE 2.
List of primers
| Primera | Homology sequenceb |
|---|---|
| nusA-Cm(f) | CCCCACTTTTAATAGTCTGGATGAGGTGAAAAGCCCGCG-cat(f) |
| nusA-Cm(r) | CGTTACATCTGTCATGCTGTTCCTTCCTGCTACAGTTTA-cat(r) |
| nusA-pCR(f) | GCTGCCCGTAATATTTGCTGGTTCGGTGACGAAGCGTAA-pCR(f) |
| nusA-pCR(r) | ATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTT-pCR(r) |
| nusA-check(f) | TAAAGATGAAGTGTTCGCGCTGAG |
| nusA-check(r) | GCTGTACCAGGCGTTCCACGGAGG |
| nusB-Cm(f) | TGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCC-cat(f) |
| nusB-Cm(r) | CATGGAACGGTCTTCCGTGAATCTACCGGCCTGGATATCA-cat(r) |
| nusB-check(f) | AAGGTGCAGAAGCTGCACTGACCGCGC |
| nusB-check(r) | GGCAATCAGGGAGAACTCGCCACATGC |
| nusE-Km(f) | AATCATTTTCGTTTATAAAATAATTGGAGCTCTGGTCT-kan(f) |
| nusE-Km(r) | CAATCATTGTTTCAACCTCTCAATCGCTCAATGACCTGA-kan(r) |
| nusE-exp(f) | CAGAAGGAGATATTCATATGCAGAACCAAAGAATCCGTATC |
| nusE-exp(r) | TTGCATGCGCATCTAGATTAACCCAGGCTGATCTGCACGTC |
| nusE-check(f) | CTCCCATCAATCGTAATGGGTCTG |
| nusE-check(r) | ACGGGTCATACCCACTTTTTTACC |
| nusG-Cm(f) | GGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTT-cat(f) |
| nusG-Cm(r) | TTGTGCAACGATTAAATCGCCGCTTTTTTGATCGCTGGG-cat(r) |
| nusG-check(f) | CGCAGTAATGTCACTGATCCTGTG |
| nusG-check(r) | GCGAAATTGTATTCCAATCTCACG |
| rho-Km(f) | ACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACT-kan(f) |
| rhoL-Km(f) | TAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAA-kan(f) |
| rhoL-KmSD | rhoL-Km(f)-CAGGATGAGGATCGTTTCGCATG |
| rho-Km(r) | AGCAAAACGCCACGTAAACACGTGGCGTTTTTGGCATAAGACAAA-kan(r) |
| rho-check(f) | TGTTGACTTCGTATTAAACATACC |
| rho-check(r) | GATGAGATCAGTACTCACTGTCAG |
| rmf-Ap(f) | TTTCTTTTCCACCAGAAACCAGTATGAGGGAAACGAGGC-amp(f) |
| rmf-Ap(r) | TCCTCCGCAATGCGGAGGTTTCTTTTTAAAGAGACAGAA-amp(r) |
| rmf-check(f) | TGACGGCAGTTATGATTCGCGGTA |
| rmf-check(r) | AGGATACGTCTGCCTTCTGATTAT |
| rpsV-Km(f) | TGTTGTCCTTAAAACTAGCTACAGGATTGAGGAGTTAAA-kan(f) |
| rpsV-Km(r) | AATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAA-kan(r) |
| rpsV-check(f) | TTATGTGGTCAGTGGCCAGCACC |
| rpsV-check(r) | TTGGTTCCATGTCACTCACTCTT |
| yfiA-Km(f) | GCTGAATTCACCAAGACGGGAAGACAAGAGGTAAAATTT-kan(f) |
| yfiA-Km(r) | CGCCCGAAGGCGCGTTGGCGATACACTCAATATAAAGGA-kan(r) |
| yfiA-check(f) | CACATTTTGACATCAGGAACGG |
| yfiA-check(r) | GTACTGTTTTCACGCTGTCAAC |
| yhbH-Cm(f) | AACAACTCGTTTGACCCAACCGATAAGGAAGACACT-cat(f) |
| yhbH-Cm(r) | AACAGACCGCCATGCACATGCTAATTGCCCGGACAA-cat(r) |
| yhbH-check(f) | CACTGTTGCGAAGTACCGAGAGTCT |
| yhbH-check(r) | ATTAACCGTACAAATGGCCCGTTGT |
| amp(f) | ATGAGTATTCAACATTTCCGT |
| amp(r) | TTACCAATGCTTAATCAGTGA |
| cat(f) | ATGGAGAAAAAAATCACTGGATATAC |
| cat(r) | TTACGCCCCGCCCTGCCACTCATC |
| kan(f) | ATGATTGAACAAGATGGATTG |
| kan(r) | TCAGAAGAACTCGTCAAGAAG |
| pCR(f) | CCTGAATTCTGCAGATATCCATCAC |
| pCR(r) | CATAGCTGTTTCCTGTGTGAAATTG |
a
f and r indicate forward and reverse primers, respectively.
b
Sequences of primers used to generate gene cassettes for recombineering are shown in two parts. The first part is shown in normal font and represents sequences complementary to the flanking regions of the gene of interest. The second part is in bold and was used for amplification of the respective PCR fragments; the sequences for these primers are shown at the bottom of the table.