TABLE 5.
Glucose metabolism genes of B. cereus F4430/73 differentially expressed in the fnr mutant strain grown under aerobic and anaerobic nitrate conditionsa
Gene | Difference in mRNA level (n-fold) under the indicated conditionsb
|
|
---|---|---|
Without O2, with nitrate | With O2 | |
Genes for glucose catabolism | ||
ptsG | +1.0 | −2.5 |
ptsH | −1.2 | −4.1 |
pykA | −2.4 | +8.1 |
pykF | −1.7 | +8.6 |
pckA | +1.4 | −13.3 |
pycA | −1.1 | −2.8 |
ldhA | +1.0 | +24.1 |
ldhB | +1.3 | +3.2 |
ldhC | +1.1 | −1.1 |
pfl | −1.3 | −3.5 |
pdhA | +1.3 | +2.8 |
pta | +1.1 | +1.5 |
ackA | +1.2 | +1.3 |
adhE | −1.3 | +26.6 |
adhA | −1.4 | +10.7 |
sdhA | +1.2 | +34.0 |
Genes for nitrate respiration | ||
yutJ | −1.6 | −1.8 |
yjld | +1.6 | −2.5 |
yrkE | −1.3 | +2.3 |
yumB | +1.5 | −2.5 |
narG | −1.2 | +2.2 |
nasD | −1.4 | +2.8 |
Regulator | ||
resD | −1.2 | +1.5 |
Cells were grown in pH-regulated batch cultures on MOD medium with 30 mM glucose as the carbon source, and 20 mM nitrate was used if needed.
Each value represents the mean specific level of the mRNA in the mutant sample in relation to that in the wild-type sample. Data are presented as described in Table 4 footnote b.