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Like CcdBF, the CcdBO157 toxin targets the DNA gyrase
| Strain | Efficiency of transformationa
|
||
|---|---|---|---|
| pBAD33 | pBAD-ccdBF | pBAD-ccdBO157 | |
| SG22622 | 1.1 | 6.7 × 10−5 | 7.1 × 10−5 |
| SG22622 gyrA462 | 1.1 | 0.9 | 1 |
Efficiency of transformation was calculated as the ratio of the number of transformants obtained on 1% arabinose plates to the number of transformants obtained on plates without arabinose. This experiment was performed at least in triplicate.
