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Purification of 2-amino-5-chloromuconate deaminase from Comamonas sp. strain CNB-1
| Step | Total protein (mg) | Total activity (μmol·min−1) | Sp act (μmol· min−1·mg−1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Cell extract | 179 | 453 | 2.5 | 1.0 | 100 |
| (NH4)2SO4 | 79 | 358 | 4.5 | 1.8 | 79 |
| Superdex 200 | 0.71 | 26 | 36.6 | 14.6 | 5.8 |
| Mono Q HR5/5 | 0.23 | 15 | 65.3 | 25.7 | 3.3 |
