TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| S. aureus strains | ||
| RN4220 | Laboratory strain; rsbU | 19 |
| RN6390 | Parental strain of KB350 | 33 |
| KB350 | RN6390 cidA::Erm Ermr | 37 |
| WCUH29 | Clinical human isolate; rsbU+ | 18 |
| 15981 | Clinical human isolate | 46 |
| ΔarlRS | 15981 with deletion of arlRS | 46 |
| RN4220/Pspac-gcp | spacp-regulated gcp mutant | 49 |
| RN4220/gcp-as | TetR-regulated gcp antisense mutant | 49 |
| WCUH29/gcp-as | TetR-regulated gcp antisense mutant | This study |
| ΔarlRS/gcp-as | TetR-regulated gcp antisense mutant | This study |
| Plasmids | ||
| pYH4 | Shuttle vector; Ermr | 49 |
| pYH4/gcp-as | Shuttle vector with gcp antisense construct; Ermr | 49 |
| pSB2025 | Shuttle vector containing luxABCDE; Cmr | 34 |
| pCY806 | pYH4/gcp-as with promoterless luxABCDE; Ermr | This study |
| pLZ106 | pCY806 with cidA promoter-lux reporter fusion; Ermr | This study |