TABLE 2.
Primers used in this study
| N315 open reading frame | Gene | Primer orientation | Primer sequence (5′-3′)a |
|---|---|---|---|
| SA1854 | gcp | Forward | TTGGCGCGCCGTTTAAAGCTATTCAA |
| Reverse | GGGATAGTTAATTGAATGTC | ||
| SA1248 | arlR | Forward | TGACAAAGTTGCTGGGCTTGATTAC |
| Reverse | TGTGGCTGACGACGTAAAATTGC | ||
| SA0252 | lrgA | Forward | TGAAACAACAAAAAGACGCATCAAAACCAG |
| Reverse | ACTTCGCCTAACTTAACAGCACCAG | ||
| SA0250 | lytS | Forward | GCATGGTTCTATCGTCGGTACATTG |
| Reverse | ACTTACTTTGCGTTTCGGCTTCAC | ||
| SA2329 | cidA | Forward | GTCTTTTTCTTCATACCGTCAGT |
| Reverse | TCATTCATAAGCGTCTACACCT | ||
| SA0641 | rat/mgr | Forward | CGCAGATGATTATATGCAAAAC |
| Reverse | CGACAGCATCTTGCCAAGTC | ||
| SA0905 | atl | Forward | GCTGGTTATAGTTTAGTTGATGATG |
| Reverse | GGTTGTGCTGAAGCGCTAAAAG | ||
| SA0265 | lytM | Forward | GCAGGAGATAACAATGACTACAC |
| Reverse | TTACTTGCTGATCCACCATTTTG | ||
| SA1090 | lytN | Forward | AGCTGAACCTGGGGACTTAG |
| Reverse | CAACTTTATGTGCAACCTCTGC | ||
| SA1206 | femA | Forward | TCATCGATTACAGACGAAGACAC |
| Reverse | TCTTTTAGTTTAGACGGCGCAACC | ||
| 16S rRNA | Forward | CTGTTGCACATCTTGACGGTA | |
| Reverse | TCAGCGTCAGTTACAGACCA | ||
| lux | Forward | TAGAATTC GCGGCCGC GGCCGGCC AGTACT AGGTAGGTAATAGGAGGACTCTCTATGAAATTTGGAAACTT (EcoRI, NotI, FseI, and ScaI, respectively) | |
| Reverse | TAGAATTCTCAACTATCAACGCTTCGGTTAAGCT (EcoRI) | ||
| SA2329 | cidA promoter | Forward | AAGCGGCCGCCCACTTTGCCAGCTGATCATCA (NotI) |
| Reverse | ACAGTACTAAATATGTCTAAAATGTTACAATAACT (ScaI) | ||
| SA0905 | atl promoter | Forward | AAGCGGCCGCTGATTTTGTTAGCATGTGGAGGAA (NotI) |
| Reverse | TTAGTACTTGTGCGTATTTAACCAATTTGTA (ScaI) |
a
Restriction sites are indicated in bold, with the corresponding enzyme(s) shown in parentheses after the sequence.