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. 2007 Feb 9;189(8):3099–3105. doi: 10.1128/JB.01753-06

TABLE 1.

Kinetic parameters with amino acid as the only nutrient sourcea

Parameter Variable for indicated strain with noted incubation time
E. coli ATCC 25922
C. freundii ATCC 8090 (1.5 h)
1.5 h 4.5 h
Km (μM) 15.0 ± 7 223.6 ± 51.8 14 ± 2
KAA (μM) 13.5 ± 7 221.8 ± 49.7 10 ± 2
IntAA (μM) 1.5 ± 1.2 1.8 ± 0.5 3.8 ± 3
β-galAA=0 (% of Vβ-galmax) 5 ± 3 0.37 ± 0.32 14 ± 1
Vβ-galmax (10−8× U/cell)b 1.0 ± 0.4 40.3 ± 7.0
μmax (h−1) 0.94 ± 0.08
KAA-μc (μM) 122.3 ± 33.2
a

The experimental points are expressed as means ± SD. See the legends for Fig. 1, 3, and 4a and b for the procedure and the text for the description of parameters.

b

Starting cells, i.e., the number of cells at the beginning of the experiment.

c

Michaelian constant relative to the mean growth rate.