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Substitutions at the N terminus decrease ZntR stability. (A) The t1/2s of several N-terminally six-His-tagged ZntR variants with amino acid substitutions in the DNA binding motif or in the residues known to coordinate zinc. wt, wild type. (B) Schematic representation of ZntR domains (based on UniProtKB/Swiss-Prot prediction and reference 5). (C) Illustration of the N-terminally six-His-tagged variants of ZntR carrying substitutions at the C terminus and their t1/2s (in minutes).
