TABLE 2.
Oligonucleotide primers used in this study
| Name | Sequence (5′-3′)f | Restriction site | Reference |
|---|---|---|---|
| 7350F | GAGCTGCGCAAGGAACTCAa | This study | |
| 7350R | TGGGTAAAGGACGCGGATAACa | This study | |
| bviIKOF | GTCCGAGGATCCAGAGCGb | BamHI | This study |
| bviIKOR | GCACGCGAAGCTTCACGGb | HindIII | This study |
| bviIproB | CGAATTGGATCCATTATCGGb | BamHI | This study |
| bviIproX | AATAGCCCTCGAGTGGCCb | XhoI | This study |
| bviRKOF | CACGGTCGTCTAGACGAGGb | XbaI | This study |
| bviRKOR | GGCCGAAGCTTGATGAATCGb | HindIII | This study |
| bviRUF | GACCAGCTCGAGGTAGCCG3b | XhoI | This study |
| bviRUR | CAGTGGTCGGATCCGAGCGb | BamHI | This study |
| cepIF | CAGGCGGCGATAGCTTGc | This study | |
| cepIR | CACAGATCCGAGGACATCCAc | This study | |
| cepRF | GAGAAAGAATGGAACTGCGCc | This study | |
| cepRProF | GGCCGCTCGCGACATGGTc | This study | |
| cepRProR | CCGCGGCGCTGAATTGTTGGc | This study | |
| cepRR | TTGTTCACGTGGAAGTTGACc | This study | |
| ExcepI | GCCTGCAGGGCACAACGACGCCTATCATGCc | 33 | |
| G4cepIF | TCAATCCCGCCGATCAAGe | This study | |
| G4cepIR | GCGCGAAAGACCTGAGACTGe | This study | |
| G4cepRHind | CATTTCAAGCTTGAGCTGGACCa | HindIII | This study |
| G4cepRKOF | CGGATCGGTACCTTGGGATGa | KpnI | This study |
| G4cepRKOR | CCGCAAGCTTCCCGTTTCACa | HindIII | This study |
| IN7349F | TCAAGACCCAGCATCTCAATGa | This study | |
| IN7349R | ACCCGACAGGTTGATGAGCa | This study | |
| IN7350F | TCCAACGGACCGGTATGTGa | This study | |
| IN7350R | AGGCCACGAACGGAGAGGTAa | This study | |
| INcepI | GCGGATCCACCAGACGCCCATCTACCTGCTTCGc | 33 | |
| R2Cla3RE | GAACGAAGGTCTGCATGGATGc | This study | |
| RTbviIF | CACGGAGAACGCAATGAGGb | This study | |
| RTbviIR | CACGCGGATACCCTTTACGTCb | This study | |
| RTbviRF | CAGACGTGGGTCGAACGCTAb | This study | |
| RTbviRR | ATAGTTGGCCGTGTTGGCGb | This study | |
| RTsigAF | AATGACCGAGGCGAACCTGa | This study | |
| RTsigAR | TCTTGTCTTCCGGCATCTCCa | This study | |
| UnicepIF | GACCTTCGTTCACGAGGAAGd | This study | |
| UnicepIR | CGTCACGCCGATCAGCTGCd | This study |
a
Primer designed based on the sequence from the incomplete B. vietnamiensis G4 sequencing project (http://genome.jgi-psf.org/draft_microbes/bur08/bur08.home.html).
f
Restriction endonuclease sites incorporated into primers are underlined.