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. 2007 Feb 2;189(8):3006–3016. doi: 10.1128/JB.01544-06

TABLE 3.

Summary of AHL production, bviIR gene presence, and bviIR expression in clinical and environmental B. vietnamiensis strainsi

Strain Source of isolate HHL productiona OHL productiona DHL productiona Presence of bviIR genesb Expression of bviI Expression of bviRg Presence of 7349 and 7350h
PC259 Clinical + + + + +c/−d,e +
FC466 Clinical (+) (+) + c,d,e (+) +
FC441 Clinical + + + c,d,e (+) +
C2822 Clinical + + + c,e (+)
FC369T Environmental + + + + +c,d,f ND +
DBO1 Environmental + + + + +d,f ND +
G4 Environmental + + + + +c,d,f + +
a

Production of AHLs was determined by AHL-TLC bioassays using A. tumefaciens A136(pCF218)(pCF372) as the reporter strain.

b

The presence of the bviI and bviR genes was determined by PCR.

c

The expression of bviI was assessed by RT-PCR.

d

The expression of bviI was analyzed by transcriptional analysis of bviI-luxCDABE fusion constructs (pRM455, pRM465, pRM475, pRM485, pRM495, pRM415) in their respective host backgrounds.

e

The expression of bviIG4 was analyzed by transcriptional analysis of the bviIG4-luxCDABE fusion construct (pRM465).

f

The expression of bviIPC259 was analyzed by transcriptional analysis of the bviIPC259-luxCDABE fusion construct (pRM465).

g

The expression of bviR was assessed by RT-PCR with primers internal to bviR.

h

The presence of the 7349 and 7350 genes was determined by PCR.

i

+, positive; (+), weakly positive; −, negative; ND, not determined.