TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype/phenotype or relevant feature/genea | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM109 | F′ [traD36 proAB+lacIqlacZΔM15] recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) | 71 |
| BL21 Star (DE3) | F−ompT hsdSB(rB− mB−) gal dcm rne-131 (DE3) | Invitrogen |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| M. xanthus | ||
| DK1622 | Wild type | 30 |
| LS2500 | ΔMXAN_3852 Galr Kms | This study |
| LS2501 | ΔMXAN_5522 Galr Kms | This study |
| LS2502 | ΔMXAN_4569 Galr Kms | This study |
| LS2530 | MXAN_3852-lacZ Kmr | This study |
| LS2531 | MXAN_5522-lacZ Kmr | This study |
| LS2532 | MXAN_4569-lacZ Kmr | This study |
| MxH2129 | aglU sglK Kmr | 72 |
| SW501 | difE Kmr | 70 |
| Plasmids | ||
| pBJ113 | galK Kmr | 28 |
| pET102/D-TOPO | Expression vector; Apr | Invitrogen |
| pKY481 | lacZY Kmr | 11 |
| pAMM6 | ΔMXAN_3852 Kmr | This study |
| pAMM7 | ΔMXAN_5522 Kmr | This study |
| pAMM10 | ΔMXAN_4569 Kmr | This study |
| pAMM11 | pET with MXAN_3852 Apr | This study |
| pAMM12 | pET with MXAN_5522 Apr | This study |
| pAMM15 | pET with MXAN_4569 Apr | This study |
| pAMM18 | MXAN_3852-lacZ Kmr | This study |
| pAMM19 | MXAN_5522-lacZ Kmr | This study |
| pAMM20 | MXAN_4569-lacZ Kmr | This study |
a
Kmr, Kms, Apr, and Galr refer to kanamycin resistance, kanamycin sensitivity, ampicillin resistance, and galactose resistance, respectively.