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. 2007 Feb 9;189(8):3271–3279. doi: 10.1128/JB.01790-06

FIG. 2.

FIG. 2.

(A) Southern blotting analysis of plasmid and total DNA from various strains of X. axonopodis pv. citri. The blot was probed with a digoxigenin-labeled, 2.3-kb internal SphI fragment of apl1 (pthA homolog). Lanes 1 and 2, intact and BamHI-digested plasmid DNA from strain KC21; lanes 3 to 22, BamHI-digested total DNA from various strains; lanes 3 and 4, KC21T46 and its transformant with pLpB3.0; lanes 5 and 6, KC21T14 and its transformant with pLpthAposi3; lanes 7 to 14, less aggressive strains KC21, KC30, KC32, KC33, KC34, KC35, KC39, and KC40; lanes 15 to 22, normally aggressive strains KC15, KC17, KC18, KC20, KC22, KC24, KC25, and KC31. (B) Agarose gel electrophoresis of plasmid and total DNA from various strains of X. axonopodis pv. citri shown in A. (C) Schematic representation of Tn5 insertion into hssB3.0 and pthA-KC21 from mutants KC21T46 and KC21T14. Black boxes represent the leucine zipper-like region (LZ), nuclear location signals (NLSs), and an acidic transcriptional activation domain (AD). Restriction enzyme cleavage sites are shown with a B for BamHI and an S for SphI.