Accumulation of PAL gene transcript in the leaves of Otachibana (A) and navel orange (B) after inoculation with X. axonopodis pv. citri strain KC20 and its transformant with pLpB3.0, KC21T46 and its transformant with pLpB3.0, and KC21. Distilled water (DW) was used as the control inoculum. Total RNA was isolated from the lesions 1, 4, 8, and 12 days after inoculation and used for real-time quantitative RT-PCR. The relative mRNA level was calculated with respect to the level of the corresponding transcript in uninfected leaves of the respective plant (equaling 1). Bars represent standard errors (n = 3).