TABLE 1.
Bacterial strains and plasmids
| Bacterial strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| X. axonopodis pv. citri | ||
| KC15 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC17 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC18 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC20 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC22 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC24 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC25 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC31 | Wild-type strain; normally aggressive to Otachibana | 19 |
| KC21 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC30 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC32 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC33 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC34 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC35 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC39 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC40 | Wild-type strain; less aggressive to Otachibana | 19 |
| KC21T46 | Tn5 inserted mutant of KC21; normally aggressive to Otachibana | This study |
| KC21T14 | Tn5 inserted mutant of KC21; less virulence to citrus plants | This study |
| E. coli, DH5α | F−recA φ80dlacZΔM15 | TAKARA BIO Inc. |
| Plasmids | ||
| pSUP2021 | Suicide vector containing Tn5, 11.7 kb, ColE1 Ori; Apr Cmr Kmr | 20 |
| pLAFR3 | Broad-host-range cosmid cloning vector, 21.7 kb, RP4 Ori; Tcr | 21 |
| pUC18 | ColE1; lacZ Apr | TAKARA BIO Inc. |
| pUC19 | ColE1; lacZ Apr | TAKARA BIO Inc. |
| pNKBH1 | pBluescript clone of 13-kb DNA fragment containing apl1 from X. campestris pv. citri strain NA1 | 11 |
| pLpthAposi1 | pLAFR3 clone of 26.2-kb DNA fragment containing the 3.0-kb and 3.7-kb BamHI fragments hybridized with the 102-bp repeat unit of apl1 | This study |
| pLpB3.0 | Subclone of pLpthAposi1 containing the 3.0-kb BamHI fragments hybridized with the 102-bp repeat unit of apl1 | This study |
| pLpthAposi3 | pLAFR3 clone of 25.3-kb DNA fragment containing the 3.3-kb BamHI fragments hybridized with the 102-bp repeat unit of apl1 | This study |
| pLpthAposi5 | pLAFR3 clone of 22.1-kb DNA fragment containing the 3.1-kb BamHI fragments hybridized with the 102-bp repeat unit of apl1 | This study |