FIG. 2.
Time-dependent increase in phage titer as a consequence of added (A) salt and (B) salt and mitomycin C. Phage-containing supernatants were obtained from MG1655 cells lysogenized with λimm434 bacteriophage grown under the following conditions. For the experiments shown in panel A, stationary-phase cells were washed, suspended in rich medium without (gray bars) or with (black bars) 200 mM added NaCl, and grown for the indicated times as described in Materials and Methods. For the experiments shown in panel B, cells were treated exactly as for panel A, except that 3.5 μg/ml mitomycin C was added to the growth medium after the cells were washed. The data are plotted as the logarithm of the increase in PFU per viable cell from time zero to the indicated time.