TABLE 1.
Primers used to amplify the unknown genomic region surrounding the PPTNs gene
| Primer | Sequence (5′-3′) |
|---|---|
| Panhandle Roko1 | GTGGTAAACCAGTATTAGCGG |
| Panhandle Roko2 | GTCTAGTCCACAAGGCTTTAAG |
| Panhandle Roko3 | CCATTGTTATTCCTGGTC |
| Panhandle Roko4 | CATTAAGGCCGCATAACCGAT |
| Panhandle Roko5 | GCATCGTGAATCACAGTGCC |
| Panhandle Roko6 | CAATAGTAGTGGCGTGGCAA |
| Panhandle Roko7 | CTAAATGGCCAGCATAAGCA |
| Panhandle Roko8 | GCTTATGCTGGCCATTTAG |
| Panhandle Roko9 | GATGAATAGCCGGGTCAAG |
| Panhandle Roko10 | GCGTGATGCTGTAAATGC |
| Panhandle Roko11 | CTTGACCCGGCTATTCATC |
| Panhandle Thorne1 | GTGATAATTCCCGGCACACAGAGC |
| Panhandle Thorne 2 | CCGTTATTGCACTTGTAAAGAGG |
| T7P Adapter | CCCCTATCCACCCTTACACCTATC |
| T7Pr2 Adapter | CCTTACACCTATCCCTCCGTAATAC |