TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli strains | ||
| EP432 | melB(LiDa) nhaA1::kan ΔnhaB1::cam ΔlacZY thr-1 | 47 |
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Gibco-BRL |
| KNabc | TG1 (ΔnhaA ΔnhaB ΔchaA) | 43 |
| Plasmids | ||
| pMW118 | Cloning vector; Ampr | NipponGene, Toyama, Japan |
| pMWBSMrp | pMW118(+) derivative; contains the full mrp operon from B. subtilis | 21 |
| pMWOF4Mrp | pMW118(+) derivative; contains the full mrp operon from B. pseudofirmus OF4 | 21 |
| pMWSaMnh | pMW118(+) derivative; contains the full mnh operon from S. aureus | This study |
| pMWSaMnh2 | pMW118(+) derivative; contains the full mnh2 operon from S. aureus | This study |
| pGEM-3zf | Cloning vector; Ampr | Promega |
| pGEMBs-Mrp | pGEM-3Zf(+) derivative; contains the full mrp operon from B. subtilis | This study |
| pGEMBpOF4-Mrp | pGEM-3Zf(+) derivative; contains the full mrp operon from B. pseudofirmus OF4 | This study |
| pGEMMnh | pGEM-3Zf(+) derivative; contains the full mnh operon from S. aureus | This study |
| pGEMMnh2 | pGEM-3Zf(+) derivative; contains the full mnh2 operon from S. aureus | This study |
| pBR322 | Cloning vector; Ampr | New England Biolabs |
| pGM36 | pBR322 derivative; contains the full nhaA gene from E. coli | 12 |
a
LiD, Li+ dependent.