TABLE 2.

PCR primers

Primer	Sequence	Use	Expected size (bp)
OAG 217	5′-GCT CGC AAG AGC CGT CGA TGC C-3′	Insertion mutation in mspA gene	294
OAG 218	5′-GCC GGA GGT GAC TGT GGA TGT C-3′
OAG 213	5′-TCG CCA CCA TCG CCA TGC TCG G-3′	Insertion mutation in mspB gene	483
OAG 214	5′-TGT CCT GGT TGA TGG ACG AGT C-3′
OAG 215	5′-AAG GCC GCC AAG GAC GTG ACA G-3′	Insertion mutation in mspC gene	262
OAG 216	5′-CGA CCT CAT CGC CCT CCA ACT G-3′
OAG 492	5′-GGA GAT TCG TGC GTT GGA TGC G-3′	mspA mutation verification	5,610
OAG 493	5′-GAG CAT CTC TGT CGG CAA CGT G-3′
OAG 488	5′-GTC TCC ACC AGC ATC TCC AGC A-3′	mspB mutation verification	5,899
OAG 489	5′-CTC ACG TTG AGC AGC ATG GCG A-3′
OAG 490	5′-ACG ACA CGA GCT TCG ACC GCA T-3′	mspC mutation verification	5,169
OAG 491	5′-CCA CAG TCG TCG ATT CAC CGC T-3′
OJD2U	5′-CAT CGA TGC AGC ATC CAC CC-3′	RT-PCR for mspA expression	170
OJD2D	5′-CTC ATC CTT GAT GAA GAC C-3′
OJD10U#2	5′-CAA CAA CCA CAG GAG AAC CTG-3′	RT-PCR for mspB expression	313
OJD10D	5′-GTC GAC GAA GAT CTT GTC GC-3′
OJD9U	5′-GTG ACA GGC ATC CAG ACG G-3′	RT-PCR for mspC expression	297
OJD9D	5′-CTG CTT CTT CTT GGA ACC C-3′
OJD4U	5′-CAA GGG AAC TGA GAG ACA GG-3′	RT-PCR for 16S rRNA	120
OJD4D	5′-CTC TAG AGA TCC ACT TGC G-3′
OJD23U-1	5′-ATG GTG GAA CTG GAA GCC G-3′	RT-PCR for MXAN_2431 gene	500
OJD23D-1	5′-GTC CTT TCG AAG TCT TGA CGG-3′
OJD24U-2	5′-CAC CGT CAT CTG TGG TGG C-3′	RT-PCR for MXAN_6970 gene	500
OJD24D-2	5′-CTT CTC GTT GAG CCA CAC CG-3′