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. 2007 Feb 16;189(8):3280–3289. doi: 10.1128/JB.01936-06

FIG. 3.

FIG. 3.

Complementation studies in yycH and yycI deletion strains. To determine whether the ΔyycH and ΔyycI strains would be complemented by expressing the respective genes from elsewhere in the chromosome, strains containing either yycH or yycI or empty pDG1664 vector integrated in the thrC locus were grown in LB broth. Shown are (A) growth curves and (B) β-galactosidase specific activity in Miller units of JH25020, a wild-type strain harboring pDG1664 vector in the thrC locus (diamonds), and ΔyycH strains JH25023 through JH25025 harboring either pDG1664 (squares), pDG1664-yycH (triangles), or pDG1664-yycI (circles) in the thrC locus, respectively. Also shown are (C) growth curves and (D) β-galactosidase specific activity in Miller units of wild-type strain JH25020 harboring pDG1664 vector in the thrC locus (diamonds) or ΔyycI strains JH25026 through JH25028 harboring either pDG1664 (squares), pDG1664-yycH (circles), or pDG1664-yycI (triangles) in the thrC locus, respectively.