TABLE 1.
Pseudomonas strains and plasmids used
| Strain or plasmida | Relevant characteristicsb | Reference or source |
|---|---|---|
| P. aeruginosa strains | ||
| PAO25 | leu arg | 22 |
| PAN1 | PAO25 xcpQ::Gm | 1 |
| PAN15 | PAN1, suppressor, LPS core deficient | This study |
| PAN16 | PAN1, suppressor, LPS core deficient | This study |
| AK1012 | PAO1, LPS core deficient | 24 |
| AK1401 | PAO1, LPS B-band deficient | 24 |
| PAN38 | AK1012 xcpQ::Gm | This study |
| PAN39 | AK1401 xcpQ::Gm | This study |
| P. alcaligenes 93A | 21 | |
| Plasmids | ||
| pUC18 | Ampr, ColE1 | NCCB |
| pMMB67EH | Ampr, IncQ | 18 |
| pUR6500HE | pMMB67HE derivative, Kmr | 17 |
| pVDZ′2 | Tetr, IncP | 12 |
| pRK2013 | Kmr Tra+ Mob+ | 14 |
| pLAF600SB | xcpPQalc in PLAFR3 | 21 |
| pB28 | xcpQaer in pMMB67EH | 1 |
| pCK25 | xcpQaer in pUR6500EH | This study |
| pCK27 | xcpPalc in pMMB67EH | This study |
| pCK28 | xcpQalc in pMMB67EH | 1 |
| PCK29 | xcpPalc in pVDZ′2 | This study |
| pCK31 | xcpPQalc in pMMB67EH | This study |
| pCK32 | xcpQaer and xcpQalc in pMMB67EH | This study |
| pUWL6 | xcpQaer in pUC19 | 1 |
| pUWL15 | xcpQF497 in pUC18 | This study |
| pUWL20 | xcpQH13 in pUC18 | This study |
| pKNWQ1 | xcpQaer::Gm in suicide vector pKNG101, Smr | 1 |
a
P. aeruginosa strains PAN15, PAN16, PAN38, and PAN39 have been deposited in NCCB and are available as strains PC4313, PC4314, PC4319, and PC4320, respectively.
b
Gm, gentamicin; Amp, ampicillin; Km, kanamycin; Tet, tetracycline; Sm, streptomycin.