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. 2007 Feb 2;189(8):2967–2975. doi: 10.1128/JB.01583-06

TABLE 3.

Complementation of LPS mutant strains with xcpQ wild-type genes and chimerasa

Strain Mutationb Secretion with different xcpQ genes
None xcpQaer xcpQalc xcpQF87 xcpQF497
PAO25 None ++ ++ ++ ++ ++
PAN1 xcpQ ++ ++
PAN15 xcpQ, suppressor ++ + ++ ++
PAN16 xcpQ, suppressor ++ + ++ ++
PAN38 xcpQ, core + + + +
PAN39 xcpQ, O antigen + +
a

Secretion was analyzed on elastin plates by evaluating halo formation after 24 h of incubation and by analyzing extracellular protein profiles by SDS-PAGE. ++, secretion comparable to that of wild-type PAO25 cells; +, decreased but significant secretion; −, no significant secretion.

b

core, mutation causing a defective LPS outer core; O antigen, mutation causing a lack of O antigen.