TABLE 3.
Strain | Mutationb | Secretion with different xcpQ genes
|
||||
---|---|---|---|---|---|---|
None | xcpQaer | xcpQalc | xcpQF87 | xcpQF497 | ||
PAO25 | None | ++ | ++ | ++ | ++ | ++ |
PAN1 | xcpQ | − | ++ | − | − | ++ |
PAN15 | xcpQ, suppressor | − | ++ | + | ++ | ++ |
PAN16 | xcpQ, suppressor | − | ++ | + | ++ | ++ |
PAN38 | xcpQ, core | − | + | + | + | + |
PAN39 | xcpQ, O antigen | − | + | − | − | + |
Secretion was analyzed on elastin plates by evaluating halo formation after 24 h of incubation and by analyzing extracellular protein profiles by SDS-PAGE. ++, secretion comparable to that of wild-type PAO25 cells; +, decreased but significant secretion; −, no significant secretion.
core, mutation causing a defective LPS outer core; O antigen, mutation causing a lack of O antigen.