TABLE 3.
Adhesion of S. sanguinis SK36 to sHA in the presence of excess competing species
| Competing S. gordonii strain | % of S. sanguinis cells bound relative to control (mean ± SD) witha:
|
|
|---|---|---|
| 10-fold excess competitor | 50-fold excess competitor | |
| Control (no competitor) | 100.0 ± 11.3 | 100.0 ± 11.3 |
| DL1 (Challis) | 13.3 ± 2.6 | 3.4 ± 0.4 |
| scaA mutant (OB470) | 10.7 ± 1.0 | 3.7 ± 1.4 |
| abpAB mutant | 12.4 ± 1.3 | 4.9 ± 0.2 |
| sspAB mutant (UB1360) | 8.3 ± 1.3 | 4.4 ± 1.9 |
| cshAB mutant (OB277) | 7.5 ± 1.4 | 2.4 ± 0.2 |
| sspAB cshAB mutant (OB390) | 17.4 ± 7.8 | 4.1 ± 1.8 |
| srtA mutant | 116.0 ± 12.1** | 126.7 ± 30.8** |
| srtA-complemented strain | 26.8 ± 9.8 | 15.0 ± 5.9 |
| hsa mutant | 86.8 ± 16.7** | 61.1 ± 8.5** |
| hsa-complemented strain | 39.8 ± 6.8** | 5.5 ± 0.9 |
a
Radioactively labeled S. sanguinis SK36 cells were incubated with sHA in the presence of a 10- or 50-fold excess of unlabeled competing streptococcal cells. Numbers of attached S. sanguinis cells were then determined as described in Materials and Methods. As a control, S. sanguinis was incubated with sHA in the absence of any competing cells, and these adhesion levels were set to 100%.
**
, value is significantly higher (P < 0.01) than that for wild-type DL1.