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. 2007 Feb 9;189(8):3217–3227. doi: 10.1128/JB.01403-06

FIG. 1.

FIG. 1.

Structure of the plasmids used in this study. (A) IS1237 transposition activity detection system I. The IS1237 transposase gene cloned into pET28a between HindIII and NcoI sites is indicated by open boxes above the plasmid map. The solid arrows indicate the ORFs of the transposase gene. The mutation sites in IS1237 mutants are indicated by asterisks. The designations of plasmids having different transposase genes are indicated to the left of the corresponding transposase genes. (B) IS1237 transposition activity detection system II. An IS1237-like element (IS1237::Amp) was inserted into pETIS1, pETIS2, and pETIS3 at the SmaI site. The solid triangles indicate the IR sequence of IS1237 (IRR and IRL). The gray box indicates the inserted ampicillin resistance gene. (C) IS1237 promoter activity assay system. The shaded boxes indicate the GUS gene, and the open boxes indicate the upstream sequences flanking the GUS gene. The designations of the GUS-containing plasmids containing different upstream sequences are indicated to the left of the corresponding cloned elements.