FIG. 2.

IS1237 variants in L. xyli subsp. cynodontis. (A) IS1237 variants in the L. xyli subsp. cynodontis chromosome. The sequence and coding regions of IS1237 (orf2 and orf1) are shown. The positions of the d3 and d4 primers used to amplify the region around the deletion site of IS1237 and the position of the d5 primer used to amplify the entire IS1237 element are indicated. The scissor symbols indicate the deletion sites used to generate two shorter version of IS1237, and the shaded region is the deletion region of IS1237. The underlined sequence is the repeated sequence. IS1237d1-L and IS1237d1-R indicate the left and right sites at which IS1237 turns to IS1237d1; IS1237d2-L and IS1237d2-R indicate the left and right sites at which IS1237 turns to IS1237d2. (B) TA-IS1237 fusion variant in L. xyli subsp. cynodontis native plasmid pCXC100. The symbols used for the truncated IS1237 element are the same as those described above, and the truncated toxin-antitoxin cassette and the putative promoter upstream of the truncated IS1237 element are also shown. The positions of the d4 and d7 primers used to amplify the TA-IS1237 fusion variant from the chromosomal DNA of L. xyli subsp. cynodontis are indicated.