FIG. 6.

(A) Comparison of IS1237 downstream promoter (Pd) and IS1237 upstream promoter (Pu) with a strong L. xyli subsp. cynodontis promoter, PIII. The arrows indicate the IR sequence. The direct repeat sequences are double underlined. The shaded sequences are the conserved motifs between IR sequences. The potential transcription start sites are indicated by asterisks. (B) RT-PCR to detect transcription of the GUS gene. In the RT reaction we used primer GUS7. Lanes 1 and 3, RT-PCR results for Pd obtained using 5′ PCR primers Pd1 and Pd2, respectively; lanes 2 and 4, negative controls; lanes 5 and 7, RT-PCR results for Pu obtained using 5′ PCR primers Pu1 and Pu2, respectively; lanes 6 and 8, negative controls; lane M, marker. The results demonstrate that Pu and Pd can promote transcription of the GUS gene.