TABLE 1.
Plasmids used in this study
| Plasmid | Phenotype | Marker(s) | Source or reference |
|---|---|---|---|
| Vectors | |||
| pMD18-T | E. coli TA cloning vector | Ampr | Takara |
| pBR325 | E. coli cloning vector | Ampr Tcr | Lab collection |
| pCXC100 | L. xyli subsp. cynodotis native 51-kb plasmid | 25 | |
| pBE2 | E. coli-B. subtilis shuttle vector | Kmr | 12 |
| pEC-K18mob2 | E. coli-C. glutamicum shuttle vector | Kmr | 20 |
| pBL121 | E. coli cloning vector containing GUS gene | Kmr | Lab collection |
| pET28a | E. coli expression vector | Kmr | Lab collection |
| pBAD33 | E. coli cloning vector | Cmr | 13 |
| Clones | |||
| pLC011 | E. coli-L. xyli subsp. cynodotis shuttle vector, pBR325 containing the 51-kb replicon of pCXC100 | Ampr | This study |
| pUCISD | pMD18-T vector containing IS1237 in the HindIII-to-EcoRI direction | Ampr | This study |
| pUCISR | pMD18-T vector containing IS1237 in the EcoRI-to-HindIII direction | Ampr | This study |
| pUC-GUS | pMD18-T vector containing GUS gene | Ampr | This study |
| pUCISD-GUS | pUCISD containing GUS gene at the EcoRI-XbaI site; IS1237 and GUS gene have the same orientation | Ampr | This study |
| pUCISR-GUS | pUCISR containing GUS gene at the EcoRI-XbaI site; IS1237 and GUS gene have opposite orientations | Ampr | This study |
| pUC9-GUS | pUC-GUS vector containing 850-bp L. xyli subsp. cynodotis chromosome fragment before GUS gene | Ampr | This study |
| pLCISD-GUS | pLC011 vector containing the IS1237-GUS cassette from pUCISD-GUS | Ampr | This study |
| pLCISR-GUS | pLC011 vector containing the reverse IS1237-GUS cassette from pUCISR-GUS | Ampr | This study |
| pLC9-GUS | pLC011 vector containing 850-bp L. xyli subsp. cynodotis chromosome fragment before GUS gene | Ampr | This study |
| pLCP-GUS | pLC011 vector containing the L. xyli subsp. cynodotis strong promoter PIII before GUS gene | This study | |
| pBEISD-GUS | pBE2 vector containing the direct IS1237-GUS cassette from pUCISD-GUS | Kmr | This study |
| pBEISR-GUS | pBE2 vector containing the reverse IS1237-GUS cassette from pUCISR-GUS | Kmr | This study |
| pBE9-GUS | pBE2 vector containing 850-bp L. xyli subsp. cynodotis chromosome fragment before GUS gene | Kmr | This study |
| pBE2-GUS | pBE2 vector containing GUS gene after the SP6 promoter | Kmr | This study |
| pECISD-GUS | pEC-K18mob2 vector containing direct IS1237-GUS cassette from pUCISD-GUS | Kmr | This study |
| pECISR-GUS | pEC-K18mob2 vector containing reverse IS1237-GUS cassette from pUCISR-GUS | Kmr | This study |
| pEC-GUS | pEC-K18mob2 vector containing GUS gene | Kmr | This study |
| pETIS1 | pET28a vector containing the wild-type IS1237 transposase gene | Kmr | This study |
| pETIS2 | pET28a vector containing 4-bp insertion at position 230 of IS1237 transposase gene | Kmr | This study |
| pETIS3 | pET28a vector containing 1-bp insertion at position 239 of IS1237 transposase gene | Kmr | This study |
| pETIS1-IR | pETIS1 containing IS1237-like element | Kmr | This study |
| pETIS2-IR | pETIS2 containing IS1237-like element | Kmr | This study |
| pETIS3-IR | pETIS3 containing IS1237-like element | Kmr | This study |
| pBADIS | pBAD33 vector containing whole IS1237 sequence | Cmr | This study |