d3 |
GGTCACTTGTTGGCGGCG |
Amplification of partial sequences of IS1237
|
d4 |
CGAGCGCGGTGATCGTTG |
Amplification of partial sequences of IS1237
|
d5 |
GAGGTGGTTTCAGTAGTC |
Amplification of IS1237 from L. xyli subsp. cynodotis chromosome |
d12-5′ |
GTCCCATGGCGACGCTGGCTG |
Amplification of the IS1237 transposase gene |
d12-3′ |
CCCAAGCTTGTTTACCCAGTC |
Amplification of the IS1237 transposase gene |
d13 |
GGACCTAACCCCACGGATC |
Amplification of downstream flanking sequence of IS1237 in L. xyli subsp. cynodotis chromosome |
d14 |
CTCGTGGAACCTGTGTTAGAC |
Amplification of downstream flanking sequence of IS1237 in L. xyli subsp. cynodotis chromosome |
d15 |
CTCGTCGCTCTAGCAATCG |
Amplification of downstream flanking sequence of IS1237 in L. xyli subsp. cynodotis chromosome |
d16 |
CACTTACGGAGTCCAGGCAG |
Amplification of upstream flanking sequence of IS1237 in L. xyli subsp. cynodotis chromosome |
d17 |
CCGCTTCGGACCATTCACGC |
Amplification of upstream flanking sequence of IS1237 in L. xyli subsp. cynodotis chromosome |
d18 |
CACAACATATCCGTAATGAACCG |
Amplification of upstream flanking sequence of IS1237 in L. xyli subsp. cynodotis chromosome |
d48 |
CGGCCATGGTGTGGGAGC |
Construction of the mutant transposase gene |
d49 |
CGTGGAGAGAGCTCCC |
Construction of the mutant transposase gene |
d50 |
GGGAGCTCTCTCCACG |
Construction of the mutant transposase gene |
d51 |
TAAAAGCTTGTTTCAGTAGTCGTT |
Construction of the mutant transposase gene |
AMP1 |
AAGGGATCCGTGATACGCC |
Amplification of the ampicillin resistance gene |
AMP2 |
GAAGGATCCTCACCTAGATC |
Amplification of the ampicillin resistance gene |
Tn5564-p1 |
ACATCAACGGCCCGGAATAC |
Amplification of the ISLxc2 sequence |
Tn5564-p2 |
GCAACAGCCTCTCGTGGGAC |
Amplification of the ISLxc2 sequence |
Tn5564-p3 |
ACTGGAACAAGGCGAGAGGGAC |
Amplification of the ISLxc2 sequence |
Tn5564-p4 |
CATAAGGCCCGGCAACTACA |
Amplification of the ISLxc2 sequence |
Tn5564-p5 |
CAGGGTGCTCTCCGACAACG |
Amplification of the ISLxc2 sequence |
Tn5564-p6 |
CAGGGAGAGCGGAACAAGGTC |
Amplification of the ISLxc2 sequence |
AD1 |
NTCGA(G/T)T(A/T)T(G/C)G(A/T)GTT |
Arbitrary degenerate primer |
AD2 |
NGTCGA(G/C)(A/T)TGANA(A/T)GAA |
Arbitrary degenerate primer |
AD3 |
(A/T)GTGNAG(A/T)ANCANAGA |
Arbitrary degenerate primer |
AD4 |
AG(A/T)GNAG(A/T)ANCA(A/T)AGG |
Arbitrary degenerate primer |
GUS7 |
CCACACTTTGCCGTAATG |
RT-PCR primer for the GUS gene |
GUS8 |
CACAAACGGTGATACGTAC |
RT primer for the GUS gene |
AAP |
GGCCACACATCGACTAGTACGGGIIGGGIIGGGII |
5′ RACE abridged anchor primer |
AUAP |
GGCCACGCGTCGACTAGTAC |
Abridged universal amplification primer |
Pu1 |
ACAACCACCGCCACGC |
RT-PCR primer for Pu |
Pu2 |
CCGCTAACCAGCCACAA |
RT-PCR primer for Pu |
Pd1 |
CTGAAACCACCTCATCTC |
RT-PCR primer for Pd |
Pd2 |
CAAAAACGACTACTGAAAC |
RT-PCR primer for Pd |