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Overproduction of YjbI and YjbH in B. subtilis cells. Immunoblot analysis of cell extracts (10 μg total protein loaded in each lane) from different B. subtilis strains, using YjbI or YjbH antiserum, is shown. The blots were overexposed to demonstrate the presence of small amounts of YjbI and YjbH antigen. Lanes: A, 1A1/pCW7; B, LUW219 (ΔyjbIH)/pCW7; C, LUW219/pCW7-yjbIH; D, LUW219/pCW7-yjbH.
