TABLE 1.
B. subtilis strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or referenceb |
|---|---|---|
| Strains | ||
| LAB2026 | trpC2 pheA1 Δhmp::cat Cmr | 17 |
| MH5081 | trpC2 pheA1 resDE::tet Tcr | 36 |
| 1A1 | trpC2 | BGSCc |
| LUW219 | ΔyjbIH::spc Spr | 18 |
| LUW222 | yjbIΩpMUTIN2mcs-yjbI′ Emr | This study |
| LUW229 | sigBΔ2::spc Spr | 18 |
| LUW260 | Δhmp::cat Cmr | LAB2026→1A1 |
| LUW261 | Δhmp::cat yjbIH::spc Cmr Spr | LUW219→LUW260 |
| LUW264 | ΔresDE::tet Tcr | 18 |
| LUW272 | ΔyjbH::spc Spr | This study |
| LUW340 | ΔnsrR::spc Spr | This study |
| LUW365 | ΔresDE::tet ΔnsrR::spc Tcr Spr | MH5081 → LUW340 |
| Plasmids | ||
| pCR-Blunt II-TOPO | Cloning vector; Kmr | Invitrogen |
| pCW7 | Expression vector capable of replication in E. coli and B. subtilis; Cmr | This study |
| pCW7-yjbI | yjbI under the Pspac promoter in pCW7 | This study |
| pCW7-yjbH | yjbH under the Pspac promoter in pCW7 | This study |
| pCW7-yjbIH | yjbIH under the Pspac promoter in pCW7 | This study |
| pDG1726 | Integration vector for B. subtilis; Spr Amr | 11 |
| pDG1727 | Integration vector for B. subtilis; Spr Amr | 11 |
| pDG148 | Expression vector capable of replication in E. coli and B. subtilis; Amr Kmr | 35 |
| pDG148-hmp | hmp under the Pspac promoter in pDG148 | This study |
| pHP13 | Expression vector capable of replication in E. coli and B. subtilis; Emr Cmr | 12 |
| pMUTIN2mcs | Integration vector for B. subtilis; Emr Amr | 38 |
| pΔnsrR1 | Kmr Spr | This study |
| pΔyjbIH1 | Apr Spr | 18 |
| pΔyjbH1 | Kmr Spr | This study |
| pYjbI2 | Insertional Emr plasmid carrying an internal part of yjbI | This study |
| pTOPOYjbIH | Kmr | This study |
a
All LUW strains are derivatives of 1A1 and thus carry the trpC2 auxotrophic marker. Amr, Cmr, Emr, Kmr, Tcr, and Spr, resistance to ampicillin, chloramphenicol, erythromycin, kanamycin, tetracycline, and spectinomycin, respectively.
b
Arrows indicate transformation and point from donor DNA to recipient strain.
c
BGSC, Bacillus Genetic Stock Center, Department of Biochemistry, Ohio State University, Columbus.