TABLE 4.
Growth of nrdA+ strains in the absence of recombination proteins
| Strain | Relevant genotype | CFU/ml (mean ± SD)a at 37°C | CFU relative to that of nrdA+ strain at 37°C | Relative CFU (mean ± SD), TdR at 2 μg/TdR at 5 μgb |
|---|---|---|---|---|
| JK607 | nrdA+ | 3.0 × 107 ± 1.5 × 107 | 1 | 1.08 ± 0.14 |
| JK625 | recA | 1.1 × 107 ± 0.4 × 107 | 0.34 | 0.41 ± 0.02 |
| JK626 | recB | 7.5 × 106 ± 3.3 × 106 | 0.25 | 0.62 ± 0.11 |
| JK706 | ruvABC | 1.3 × 107 ± 1.1 × 107 | 0.43 | 0.63 ± 0.08 |
| JK707 | recB ruvABC | 9.4 × 106 ± 3.4 × 106 | 0.31 | 1.12 ± 0.18 |
a
Individual colonies from rich medium plates were resuspended, and appropriate dilutions were plated on rich medium plates. The number of independent experiments, done on different days, was between 8 and 24.
b
Individual colonies from rich medium plates were resuspended, and appropriate dilutions were plated on M9 minimal plates containing either 2 μg/ml or 5 μg/ml of thymidine (TdR).