iTRAQ analysis of ribosomal particles isolated from E. coli MG1655 cells grown at 37°C. (A) Representative polysome trace of E. coli MG1655 grown at 37°C. Fractions representing polysomes and 30S, 50S, and 70S ribosomal particles (shown in boxes) were pooled and labeled with isobaric tags 114, 115, 116, and 117, respectively. (B) The relative ratios (30S/polysome, [black], 50S/polysome [dark gray], and 70S/polysome [light gray]) of select proteins are shown. Error bars show standard errors of the means.