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Ribosome association of select E. coli proteins. Extracts from cells expressing the indicated tagged proteins were separated by sucrose density centrifugation, and fractions were probed with relevant antibodies. The proteins were tagged with the SPA or M2 tag. The positions of the 30S, 50S, 70S, and polysome fractions, as determined by absorbance at 254 nm, are shown. The leftmost lane is a loading control (1/100 of the extract loaded).
