FIG. 5.

Deletion of potential ribosome-associated proteins results in cold sensitivity in E. coli. The isogenic wild-type BW25113 and deletion strains of nonessential candidate proteins were grown to saturation, diluted into fresh medium to an OD₆₀₀ of ∼0.1, and grown for 2 or 3 generations. Equivalent serial dilutions for each strain were plated onto LB and grown overnight at 37°C or for 3 days at 18°C.