TABLE 2.

Summary of potential ribosome-associated proteins/ribosome assembly factors analyzed in this study

Protein	iTRAQ location	Polysome locationa	Copurifying proteinsb	Mutant phenotype(s)c	Comment
NudH (YgdP)	Not found	Slightly larger than 30S	CsdA, SrmB, YcbY, RluB, RluC, and YfiF	Increased 30S and decreased 70S	(Di)nucleoside polyphosphate hydrolase
RluB (YciL)	Not found	∼40S	CspA, CspC, CspD, CspE, CsdA, NudH, RluC, SrmB, YcbY, YfiF, YhbY, and YibL	Cold sensitive, increased 30S and 50S, decreased 70S	Pseudouridine synthase
RluC (YceC)	Not found	∼40S	CspA, CspC, CspD, CsdA, NudH, RluB, SrmB, YbcJ, YcbY, YfiF, YhbY, and YibL	Cold sensitive, mild polysome defect	Pseudouridine synthase
YbcJ	Not found	Small portion with the 50S	CsdA, SrmB, YcbY, RluB, RluC, YfiF, and YibL	None	S4-like domain
YbeB	50S	50S	Large ribosomal proteins	None
YcbY	Not found	∼30S	CspA, CspC, CspD, CspE, CsdA, NudH RluB, RluC, SrmB, YbcJ, and YfiF	None	Putative methyltransferase
YfiF	Not found	∼30S	CspC, CspD, CsdA, NudH, RluB, RluC, YcbY, YhbY, and YibL	None	Putative tRNA/rRNA methyltransferase
YhbY	50S	50S	CspC, RluB, RluC, YfiF, and YibL	Cold sensitive, increased 30S and 50S, decreased 70S	RNA binding protein
YibL	Unclear	Slightly larger than 30S	RluB, RluC, YbcJ, YfiF, and YhbY	None

^aSelect proteins were epitope tagged and localized by immunoblotting of polysome fractions.

^bAffinity coisolation with ribosome-associated proteins (10). Some copurifying proteins of interest are listed.

^cKeio deletion collection (2).