FIG. 1.

BACTH experiments to investigate Rsd-σ⁷⁰ and Rsd-Rsd interactions. Each line illustrates an experiment in which β-galactosidase expression was measured in BTH101 cya cells containing pK-T25 and pU-T18 and derivatives. The first column lists the plasmids present in each experiment, and the extreme right-hand column lists the measured β-galactosidase activity. The central part illustrates the different proteins encoded by the plasmids. The B. pertussis adenylyl cyclase T18 and T25 domains are illustrated as ovals. Rsd fused to T18 or T25 is illustrated by hatched squares with N and C indicating the N- and C-terminal ends of fusion proteins. Similarly, σ⁷⁰ fused to T18 or T25 is illustrated by a dark-shaded rectangle. Panels A and B illustrate experiments designed to monitor Rsd-σ⁷⁰ and Rsd-Rsd interactions, respectively. Data shown are the average of six independent experiments.