. 2007 Mar 9;189(9):3489–3495. doi: 10.1128/JB.00019-07

TABLE 1.

Plasmids used in this work

Bacterial plasmid	Description	Reference
pRW50	Broad-host-range lacZ fusion vector for cloning promoters on EcoRI-HindIII fragments, contains RK2 origin of replication, encodes Tet^r	21
pACYC184	Cloning vector; Cm^r Tet^r	3
pACYC184ΔHN	Derivative of pACYC184 with HindIII-NruI fragment removed; Cm^r Tet^s	This work
pACYC-Rsd	E. coli rsd gene cloned between BamHI and SphI sites of pACYC184	16
pACYC-RsdB	Derivative of pACYC-Rsd with shorter rsd insert cloned between HindIII and SphI sites of pACYC184, unique BglII site immediately upstream of rsd start codon	This work
pU-T18	Contains B. pertussis cya gene T18 fragment, encodes Amp^r	18
pK-T25	Contains B. pertussis cya gene T25 fragment, encodes Kan^r	19