TABLE 3.

Effects of LP18 and DA63 substitutions in Rsd measured by BACTH^a

Protein encoded by pU-T18	β-Galactosidase activity (Miller units)
In BTH101 cells carrying pK-T25 derivative encoding T25-σ70
T18	71
WT Rsd-T18	341
LP18 Rsd-T18	764
DA63 Rsd-T18	77
In BTH101 cells carrying pK-T25 derivative encoding T25-Rsd
T18	75
WT Rsd-T18	370
LP18 Rsd-T18	78
DA63 Rsd-T18	319

^aβ-Galactosidase activities were measured in BTH101 cya cells carrying pK-T25 derivatives encoding a T25-σ⁷⁰ or a T25-Rsd fusion protein and pU-T18 derivatives encoding T18 Rsd-T18 fusions. Cells were grown aerobically overnight at 30°C in LB medium supplemented with kanamycin and ampicillin. Data listed are averages of five to nine independent measurements that differed by no more than 20%. WT, wild type.