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. 2007 Mar 2;189(9):3515–3524. doi: 10.1128/JB.00055-07

FIG. 5.

FIG. 5.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of purified GriR-H (A) and estimation of its molecular weight by gel filtration (B). (A) GriR-H used in this study was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Phosphorylase b (94 kDa), bovine serum albumin (67 kDa), ovalbumin (43 kDa), carbonic anhydrase (30 kDa), and soybean trypsin inhibitor (20 kDa) were used as molecular mass standards. (B) The column used was a Sephadex G-200 column (Sigma) in a fast protein liquid chromatography system. Aldolase (158 kDa) (a), bovine serum albumin (67 kDa) (b), ovalbumin (43 kDa) (c), chymotrypsinogen A (25 kDa) (d), and RNase A (14 kDa) (e) were used for generation of a calibration line.