TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| S. flexneri 2a 2457T derivatives | ||
| BS184 | mxiC::mudI1734 Kmr | 30 |
| E. coli K-12 derivatives | ||
| BL21(DE3) | F−dcm ompT hsdS gal (λ cIts857 ind1 sam7 nin5 lacUV5-T7gene1) | 53 |
| CJD1634 | BE1414/pACYC184 | 4 |
| CJD1635 | BE1414/pAF201 | 4 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tcr)] | Stratagene |
| Plasmids | ||
| pACYC184 | Cloning vector; p15A replicon; Cmr Tcr | New England Biolabs |
| pBAD33VirB | virB gene subcloned behind arabinose-inducible PBAD promoter; Apr | 4 |
| pBluescript | Cloning vector | Stratagene |
| pET22bH-NS | hns gene in pET-22b | This work |
| pET22bVirB | virB gene in pET-22b | 31 |
| pKK232.8 | Promoter cloning vector | 9 |
| pZep08 | GFP reporter plasmid | 20 |
| pZep-proicsB-2 | icsB promoter cloned into pZEP08 | This work |
| pZep-proicsB-4 | 1-bp substitution in box 1 | This work |
| pZep-proicsB-5 | First 3 bp in box 1 replaced | This work |
| pZep-proicsB-6 | Second 3 bp in box 1 replaced | This work |
| pZep-proicsB-7 | Box 1 sequence inverted | This work |
| pZep-proicsB-8 | All box 1 bases replaced | This work |
| pZep-proicsB-9 | Box 2 sequence inverted | This work |
| pZep-proicsB-10 | All box 2 bases replaced | This work |
| pZep-proicsB-11 | 3′ end-flanking CCC replaced | This work |