TABLE 2.

Oligonucleotide primers

Primer	Sequencea (5′ to 3′)	Description or use
ET1	GGCCGGATCCATGCAATCCCAAATTAGT	Cloning of icsBp into pBluescript and DNase I footprinting PCR primer
ET2	CCGGGAATTCTCAATGAAATTGCTAATT	Cloning of icsBp into pBluescript
ET3	GAAAAGCTGGGTACCGGGCC	DNase I footprinting PCR primer (hybridizes to pBluescript)
ET5	CACTTTATCTTGTGGGATTTCATGCAAAGTAGAGCACTACATATGCTCACGAGG	Box 1 inversion
ET6	CCTCGTGAGCATATGTAGTGCTCTACTTTGCATGAAATCCCACAAGATAAAGTG	Box 1 inversion
ET9	CTTGTGGGATTTCATGATGAAATGAGCACTACATATGC	Substitution of 1 bp in box 1
ET10	GCATATGTAGTGCTCATTTCATCATGAAATCCCACAAG	Substitution of 1 bp in box 1
ET11	CTTGTGGGATTTCATGATGCCCCGAGCACTACATATGC	Replacement of first 3 bp in box 1
ET12	GCATATGTAGTGCTCGGGGCATCATGAAATCCCACAAG	Replacement of first 3 bp in box 1
ET13	CTTGTGGGATTTCATGCGTAAACGAGCACTACATATGC	Replacement of second 3 bp in box 1
ET14	GCATATGTAGTGCTCGTTTACGCATGAAATCCCACAAG	Replacement of second 3 bp in box 1
ET15	CACTTTATCTTGTGGGATTTCATGCGTCCCTGAGCACTACATATGCTCACGAGG	Replacement of all box 1 bases
ET16	CCTCGTGAGCATATGTAGTGCTCAGGGACGCATGAAATCCCACAAGATAAAGTG	Replacement of all box 1 bases
ET23	CCCGGATCCTCAATGAAATTGCTA	Cloning of icsBp into pKK232.8
ET24	GGGGAATTCATGCAATCCCAAATTAG	Cloning of icsBp into pKK232.8
ET25	GGGCGGCCGCATGCAATCCCAAATTAG	Cloning of icsBp into pZEP08
ET26	GGGTCTAGATCAATGAAATTGCTA	Cloning of icsBp into pZEP08
ET29	CTTTATCTTGTGGGTACTTTAGATGAAACGAGCACTACATATG	Box 2 inversion
ET30	CATATGTAGTGCTCGTTTCATCTAAAGTACCCACAAGATAAAGTGCCTG	Box 2 inversion
ET35	CTTTATCTTGTGGGGCCCAGCGATGAAACGAGCAC	Replacement of all box 2 bases
ET36	GTGCTCGTTTCATCGCTGGGCCCCACAAGATAAAG	Replacement of all box 2 bases
ET39	CTTTATCTTGTATAATTTCATGATGAAACGAGCAC	Replacement of 3′ end-flanking C's
ET40	GTGCTCGTTTCATCATGAAATTATACAAGATAAAG	Replacement of 3′ end-flanking C's

^aBoldface indicates changes relative to the wild-type sequence.