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. 2007 Feb 23;189(9):3650–3654. doi: 10.1128/JB.00056-07

TABLE 1.

Growth stimulation of Corynebacterium strains by iron and hemoglobin

Strain/plasmid Growth (OD600) with various iron sourcesa
+Feb −Fec −Fe with hemoglobin (μg/ml)
Hbf (50) Hb (25) Hb (10) Hb (5)
CU712 wild type 4.5 <0.1 4.8 2.8 0.5 0.3
CU712 hmuOΔ 4.0 <0.1 0.14 0.10 ndd nd
CU712 hmuOΔ/pCUhmuO 3.8 <0.1 4.2 2.5 nd nd
CU712 hmuOΔ/p37hmuO 4.2 <0.1 4.1 2.2 nd nd
CU712 hmuOΔ/p1841 3.8 <0.1 0.13 0.08 nd nd
C7(−) wild type 2.1 <0.1 4.3 2.7 0.7 0.4
C7 hmuOΔ 2.2 <0.1 1.1 0.7 nd nd
C7 hmuOΔ/pCM2.6 (vector) 5.7e <0.1 1.9 0.9 nd nd
C7 hmuOΔ/pCD293 (hmuO+) 5.9 <0.1 5.5 2.6 nd nd
C7 hmuOΔ-1841Δ 2.0 <0.1 0.9 0.5 nd nd
1737 wild type 6.4 <0.1 5.8 3.5 nd nd
1737 hmuOΔ 6.8 <0.1 6.6 3.3 nd nd
a

OD600 was determined for each culture after growth for 20 to 22 h in mPGT medium. Values shown are results from a representative experiment. All experiments were repeated at least three times, and the results of each assay varied by less than 20% from the mean.

b

+Fe medium is mPGT with 1 μM FeSO4.

c

−Fe medium is mPGT with 3.6 μg/ml EDDA.

d

nd, not done.

e

C7 strains carrying pCM2.6 plasmids grow to higher densities than strains without plasmids.

f

Hb, hemoglobin.