TABLE 2.
hmuO promoter activity in Corynebacterium strains
| Strain/plasmide | LacZ activitya in:
|
||
|---|---|---|---|
| +Feb | −Fec | −Fe/Hbd | |
| C7 wt/pCP01 (C7/hmuO-lacZ) | <0.5 | 3.0 (0.7) | 112.0 (3.0) |
| CU712 wt/pPO712 (CU/hmuO-lacZ) | 46.7 (2.3) | 129.7 (11.1) | 256.6 (20.0) |
| CU712 hmuOΔ/pPO712 | 121.3 (15.5) | 196.0 (38.0) | 922.0 (120.0) |
| C7 wt/pPO712 | 10.9 (3.0) | 15.5 (3.4) | 155.3 (24.5) |
| C7chrASΔ-hrrASΔ/ pPO712 | 1.7 (0.3) | 2.6 (0.1) | 2.5 (0.3) |
a
LacZ activity was determined as described previously (24). Values represent the means (± standard deviations) of the results of three experiments.
b
+Fe, HIBTW medium.
c
−Fe, HIBTW medium with 12.5 μg/ml EDDA.
d
−Fe HIBTW medium with Hb (hemoglobin) at 140 μg/ml.
e
wt, wild type.