FIG. 8.

Biofilm formation of Salmonella serovar Typhimurium UMR1 and isogenic mutants in EAL and GGDEF-EAL domain proteins. Cells were incubated in LB medium without salt at 28°C for 48 h with shaking at 150 rpm. The five mutants that display an altered phenotype compared to wild-type Salmonella serovar Typhimurium UMR1, namely, STM1703 (1703), STM4264 (4264), STM3611 (3611), STM1827 (1827), and STM3375 (3375), are shown. (A) Adherence to the glass surface and the clumping ability observed. (B) Quantification of planktonic cells by optical density measurements from the top layer of the cultures after sedimentation of the cell clumps for 30 min. Values represent the averages of two independent experiments. Error bars indicate standard deviations.