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Determination of molecular mass of URODBs by SDS-PAGE (A) and DLS measurements (B). (A) Lanes 1 and 2, total protein from the uninduced and induced cells, respectively; lane 3, soluble protein from the induced cell; lane 4, purified hexahistidine-tagged URODBs (20 μg). Protein markers are indicated on the left. (B) The protein samples were dissolved in 10 mM Tris-HCl, 100 mM NaCl, and 10 mM β-mercaptoethanol, pH 7.0, at a concentration of 2 mg/ml.
