TABLE 4.
TPI activities measured for different strains
| Strain | Relevant genotype | Activity (nmol min−1 mg−1)a
|
|||
|---|---|---|---|---|---|
| LB | Glc | Suc | Ery | ||
| Rm1021 | Wild type | 879 ± 29 | 990 ± 55 | 942 ± 27 | 1,569 ± 117 |
| SRmA327 | tpiA | 69 ± 4 | 85 ± 9 | 92 ± 4 | 415 ± 2 |
| SRmA355 | tpiB | 615 ± 14 | 816 ± 67 | 899 ± 44 | ND |
| SRmA366 | tpiA tpiB | 6 ± 1 | 1 ± 1 | ND | ND |
a
Activities reported are the averages of three enzyme assays. Cell extracts are from cultures grown with the following: LB, Luria-Bertani broth; Glc, VMM with 15 mM glucose; Suc, VMM with 15 mM succinate; Ery, VMM broth with 15 mM erythritol. ND, not determined, due to inability to grow with the carbon source listed.