FIG. 2.

(A) Conditional lethal phenotype of strains XOA11 and XOA12 on M9 agar plates supplemented with 0.5% (wt/vol) rhamnose or 0.5% (wt/vol) glucose. XOA11 and XOA12 grow only in the presence of rhamnose, while XOA10 and SAL10 grow equally well in either media. STC280 is shown as a control mutant for a known essential gene. (B) Depletion experiments using strains K56-2(pSCrhaB2), XOA10, XOA11, and XOA12 in nonpermissive conditions. Growth was monitored every hour using a Klett-Summerson colorimeter. The figure is representative of two independent experiments with similar results.