TABLE 2.
Sequence analysis of Av at pilE
| Genotype | Frequency of pilin Ava
|
Fold difference vs parental (comparison of means) | |
|---|---|---|---|
| Median (25th-75th percentile) | Mean (SEM) | ||
| Parental | 0.11 (0.071-0.13) | 0.11 (0.012) | |
| Region 1 | |||
| pilE::mTn#9 | 0.11 (0.019-0.13) | 0.077 (0.024) | −1.3 |
| Region 2 | |||
| pilE::mTn#15 | 0* | <0.0058† | >(−17.6) |
| Region 3 | |||
| pilE::mTn#3 | 0* (0-0.038) | 0.021† (0.011) | −4.8 |
| pilE::mTn#4 | 0.036* (0-0.071) | 0.046† (0.022) | −2.2 |
| Region 4 | |||
| pilE::mTn#7 | 0.042* (0.031-0.046) | 0.038† (0.0089) | −2.7 |
| pilE::mTn#2 | 0.065* (0.043-0.084) | 0.063 (0.023) | −1.6 |
| pilE::mTn#6 | 0.021* (0.010-0.052) | 0.035 (0.025) | −2.9 |
| Promoter mutant | 0.071 (0.071-0.13) | 0.11 (0.021) | +1.1 |
| Deletions | |||
| ΔRS1 | 0.11 (0.073-0.11) | 0.12 (0.034) | +1.2 |
| ΔRS1-PilA | 0* | <0.0057† | >(−18.1) |
a
The frequency of pilin Av at pilE was determined for selected mutants in each region upstream of pilE by the DNA sequencing assay for pilin Av, with modifications as described in Results and Materials and Methods (6). The parental strain is FA1090 1-81-S2 recA6, grown in 1 mM IPTG for full induction of recA and, therefore, pilin Av. Medians and means of the frequency of pilin Av are not significantly different from those of the parental strain unless indicated as follows: *, P < 0.05 (Wilcoxon rank-sum test of medians); †, P < 0.05 (Student's t test of means).